Oligodendrocyte Precursor Cells Proliferation

  • Presentation


    Multiple Sclerosis (MS) is a chronic demyelinating disease of the central nervous system for which there no curative treatment. Current therapies address only the inflammatory and immunological components of MS but remyelination of damaged axons appears as an attractive curative strategy. Among biological processes that may lead to remyelination of lesioned nerves include (i) the recruitment of oligodendrocyte precursor cells (OPC); and (ii) differentiation of OPC into mature myelinating oligodendroctes.

  • Compound testing


    OPC cultures are obtained from cortex of rat pups. Test compounds are added just after cellular adhesion. Proliferation is assayed by an MTT test in 96-multi well plates after 3 days.

  • Endpoints


    OPCP survival / proliferation

  • Validation data

PDGF - Metformin

    Culture of proliferating OPC.

    PDGF increase OPC proliferation with a dose dependant manner.


    References:
    Franklin R. J.M. - Why does remyelinisation fail in multiple sclerosis? (2002). Nature. 3:705-710.

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