ALZHEIMER'S DISEASE

  • Alzheimer’s disease is the most common age-related neurodegenerative disorder, characterized by the progressive degeneration of neuronal populations and the simultaneous loss of memory and cognitive functions.


    SCOPOLAMINE model

    Scopolamine, a muscarinic receptor antagonist is widely used to induce cognitive / memory impairment in clinical research (human volunteers) and in experimental animals.

  • Compound testing


    Cognitive enhancers are usually tested in this model but other treatments could also be considered. Please feel free to contact us to discuss the feasibility of your study.

  • Endpoints


    ☐ Percentage of alternation in the T-MAZE (memory performance)
    ☐ Latency in the PASSIVE AVOIDANCE test
    ☐ Recognition index in the NOVEL OBJECT RECOGNITION test

Validation data


T-Maze

  • Deficits induced by scopolamine in 3 mouse strains as assayed in the T-maze

  • Reversion of scopolamine-induced deficit

    by Donepezil (0.003 to 0.3 mg/kg) in Swiss mice in the T-maze.



  • Reversion of scopolamine-induced deficit

    by Donepezil (0.1 to 0.5 mg/kg) iin Swiss mice in the T-maze.



  • Reversion of scopolamine-induced deficit

    by Nicotine in Swiss mice in the T-maze.


  • Amphetamine :

    Reversal effect of Amphetamine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • Atomoxetine :

    Reversal effect of Atomoxetine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • Caffeine :

    Reversal effect of Caffeine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • CCMI :

    Reversal effect of CCMI on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • Galantamine :

    Reversal effect of Galantamine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • Methylphenidate :

    Reversal effect of Methylphenidate on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • Nicotine :

    Reversal effect of Nicotine on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.


  • PNU120596 :

    Reversal effect of PNU120596 on cognitive deficit induced by scopolamine in the mouse. Cognitive function was assessed by the ability of mice to perform spontaneous, continuous and sustained alternation in the T-maze.

Passive avoidance


  • Donepezil :

    Reversion of Scopolamine-induced cognitive deficit in the Passive Avoidance test in the rat.

Novel object recognition

  • Reversal of Scopolamine-induced cognitive deficit in the novel object recognition assay by Nicotine.
    Scopolamine and/or Nicotine are given at the beginning of the acquisition trial. Retention trial is performed 30 min after the acquisition trial.

    Recognition index : proportion of time spent exploring the novel object.


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