Cellular model of BRAIN INFLAMMATION
in rat GLIA-MESENCEPHALIC NEURONS culture

  • Presentation


    Neuroinflammation is now recognized as a critical process in different neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, stroke, multiple sclerosis...
    Astrocytes and microglia are key players in neuroinflammation since they release wide variety of proinflammatory mediators, including nitric oxide, cytokines and chemokines.

  • Compound testing


    Compound testing addresses the ability of test compounds to inhibit the effect of LPS on production of neuroinflammatory factors such as nitric oxide (NO) and cytokines and on neuronal death.

  • Endpoints


    ☐ NO release
    ☐ Release of proinflammatory cytokines (TNF-α and IL-1β)

    ☐ Neuronal death
NO release
  • Release of nitric oxide (NO) in LPS-activated cocultures

  • Resveratrol and dexamethasone inhibit in dose-dependent manner the NO release by LPS-activated cocultures.

TNF / IL release
  • Release of proinflammatory cytokines TNF-α and IL-1β in LPS-activated cocultures

  • Effect of resveratrol and dexamethasone on the TNF-α release induced by LPS in mesencephalic cocultures.
  • Effect of resveratrol and dexamethasone on the IL-1β release induced by LPS in mesencephalic cocultures.

Neuronal death
  • Neuronal death in LPS-activated cocultures

  • Resveratrol and dexamethasone dose-dependently inhibit neuronal death in LPS-activated cocultures.


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