ALZHEIMER'S DISEASE

  • Alzheimer disease (AD) is the most common cause of dementia. It is a neurodegenerative disease characterized by an extracellular accumulation of neurotoxic fibrillar amyloid beta peptide (Aβ).


    β-AMYLOID-INDUCED NEUROTOXICITY

    Intoxication of neuronal cultures with Aβ has been widely used to understand some of the mechanisms of cell death in AD and thus represents an instrumental in-vitro system to evaluate the efficiency of new drug candidate.

  • Model of β-AMYLOID-INDUCED NEUROTOXICITY in rat hippocampal neurons


    Compound testing addresses the ability of test compounds to inhibit Aβ (1-40) - induced cell damage in hippocampal neuron cultures.


    ☐ Dose response toxicity of Aβ

  • Aβ 1-40 intoxication using two different neuronal density per well.
  • Reduced cell viability (MTT assay) in hippocampal neuron culture in response to increasing concentration of Aβ 1-40 at a cellular density of 35 000 neurons per well.
  • Reduced cell viability (MTT assay) in hippocampal neuron culture in response to increasing concentration of Aβ 1-40 at a cellular density of 10 000 neurons per well.
  • Aβ 1-42 intoxication using two different neuronal density per well
  • Reduced cell viability (MTT assay) in hippocampal neuron culture in response to increasing concentration of Aβ 1-42 at a cellular density of 35 000 neurons per well.
  • Reduced cell viability (MTT assay) in hippocampal neuron culture in response to increasing concentration of Aβ 1-42 at a cellular density of 10 000 neurons per well.

☐ Neuroprotective effect of reference compounds against damage induced by Aβ 1-40 or -42 on primary culture of hippocampal neurons
  • BDNF protection

  • Prevention of Aβ 1-40-induced neuronal injury by pretreatment with BDNF as assessed by MTT assay in hippocampal neuron cultures
  • Prevention of Aβ 1-42-induced neuronal injury by pretreatment with BDNF as assessed by MTT assay in hippocampal neuron cultures
  • bFGF and Resveratrol protection

  • Prevention of Aβ 1-40-induced neuronal injury by pretreatment with bFGF as assessed by MTT assay in hippocampal neuron cultures
  • Prevention of Aβ 1-40-induced neuronal injury by pretreatment with Resveratrol as assessed by MTT assay in hippocampal neuron cultures

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