Human iPSC-derived Neurons

  • Presentation


    Synapse loss and dendritic atrophy are common in psychiatric and neurodegenerative disorders and they are strongly correlated with cognitive decline. For example, synaptic loss in the hippocampus or cortex is an early and invariant feature of Alzheimer's disease.
    The role of synaptic and dendritic pathology in diseases affecting the CNS highlights the importance of drug discovery strategies to promote dendrite formation and growth.

  • Compound testing


    NEUROFIT offers Human iPSC Neurons-based screening to evaluate the capability of test compound to promote dendrite formation and growth.

  • Endpoints


    A fully automated analysis of neuritogenesis in 96 well plate of primary neuron cultures, which enables the quantification of :

    Neurite total count
    Neurite total length
    Dendrite branching
Neurotrophins

  • BDNF is a member of the neurotrophin family of growth factors. BDNF acts on certain neurons of the CNS and the PNS, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses.


  • FGFs are a family of growth factors  involved in neuronal survival and differentiation during development and adulthood.


  • NGF is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of target neurons.


  • GDNF is a small protein that potently promotes the survival of many types of neurons.

Depression

  • Fluoxetine (Prozac or Sarafem), a selective serotonin reuptake inhibitor (SSRI) is the most prescribed class of antidepressant.


  • Imipramine (Tofranil or Melipramine) is tricyclic antidepressant (TCA) used to treat major depressive disorders.


  • Amitriptyline (Elavil) is tricyclic antidepressant (TCA) used to treat major depressive disorders.


  • Diazepam (Valium) is a benzodiazepin used to treat anxiety disorders.


  • Reboxetine (Endronax or Prolift), a norepinephrine reuptake inhibitor (NRI) used to treat unipolar depression.


  • Venlafaxine (Effexor, Lanvexin, Viepax or Trevilor) is an antidepressant of the serotonin-norepinephrine reuptake inhibitor (SNRI) class.

Procognitive

  • Donepezil (Aricept) is a centrally active small molecule used in the treatment of Alzheimer’s disease and different cognitive disorders.


  • Memantine is used for treating Alzheimer-type dementia. Memantine is an (NMDA)-receptor antagonist. Memantine works by blocking excess activity of glutamate.


  • Dimebon inhibits brain cell death in animal models of Alzheimer's disease and Huntington's disease. Multiple mechanisms of action: blocking the action of neurotoxic beta-amyloid proteins and inhibiting L-type calcium channels, modulating the action of AMPA and NMDA glutamate receptors.


  • Pre-084 is a sigma receptor agonist, selective for the σ1 subtype. It has antidepressant and memory enhancing effects in animal studies. PRE-084 increases the expression of GDNF.


  • 4-IBP is a σ1 Receptor Agonist.

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